3h labeling

[3H]forskolin. Direct photoaffinity labeling of the erythrocyte D-glucose transporter. Shanahan MF, Morris DP, Edwards BM. Irradiation of erythrocyte ghosts in the presence of [3H]forskolin resulted in a concentration-dependent, covalent incorporation of radiolabel into several of the major membrane protein bands. Most of the incorporation occurred in four regions of the gel. Peak 1 (216 kDa) was a sharp peak near the top of the gel in the region corresponding to spectrin. Peak 2. [3H]Inositol ([3H]Ins) labeling of phosphoinositides was studied in rat brain cortical membranes. [3H]Ins was incorporated into a common lipid pool through both CMP-dependent and independent mechanisms. These are as follows: (1) a reverse reaction catalyzed by phosphatidyl-inositol (PtdIns) synthase, and (2) the reaction performed by the PtdIns headgroup exchange enzyme, respectively. Membrane phosphoinositides prelabeled in either CMP-dependent or independent fashions were hydrolyzed by. We investigated a double-label 2-DG protocol and method of analysis in which sequential injections of 3H- and 14C-2-DG were used to map brain metabolism during two distinct experimental treatments in the same animal. In initial studies, brain sections from rats given only 3H-2-DG or only 14C-2-DG were exposed on Ultrofilm and on X-ray film with an interposed sheet of mylar (X-ray/mylar). These studies were needed to determine whether, at the 50:1 3H:14C dose ratio used, 3H-2-DG uptake would. In the present study, an in vivo assay was developed using lorazepam to fully occupy DS receptors such that [3H]Ro 15-4513 was then only able to bind to DIS receptors. When dosed i.v., [3H]Ro 15-4513 rapidly entered and was cleared from the brain, with approximately 70% of brain radioactivity being membrane-bound. Essentially all membrane binding to DS+DIS receptors could be displaced by unlabelled Ro 15-4513 or bretazenil, with respective ID50 values of 0.35 and 1.2 mg kg(-1). A dose of 30.

[3H]forskolin. Direct photoaffinity labeling of the ..

In contrast to preferential [3H]raclopride binding in layer V, a bilaminar pattern of [3H]SCH23390 labeling was observed in most cytoarchitectonic areas, with the highest concentrations in supragranular layers I, II and IIIa and infragranular layers V and VI. Whereas [3H]raclopride binding was similar in all cytoarchitectonic areas, [3H]SCH23390 exhibited some region-specific variations in the primary visual and motor cortex. The different regional and laminar distributions of D1 and D2. NSF International protects and improves global human health. Manufacturers, regulators and consumers look to us to facilitate the development of public health standards and provide certifications that help protect food, water, consumer products and the environment

Concerted CMP-dependent [3H]inositol labeling of

Studies of cell division frequently use tritiated thymi- dine ([3H]Tdr) to label mitotic cells. [3H]Tdr has been used to examine retinal histogenesis in both mammalian [1, 18] and non-mammalian vertebrates [3,6-11, 13, 15], and in birds [4]. In primates, 0.1% of the injected [3H]Tdr remains in the circulation 30 min following an intra- venous injection [14]. [3H]Tdr clearance in rodents oc- curs more slowly [2, 5]. Retinal histogenesis in non-mammalian vertebrates such as goldfish. For example, [3H]GR 65630 labels both a high and low affinity binding site (Kilpatrick et al., 1987). By contrast, [3H]quipazine and [3H]ICS 205-930 (Hoyer and Neijt, 1987) appear to label only a single population of binding sites which appear to correspond to the high affinity site labeled by [3H]GR 65630. A direct comparison between the radioligand binding properties of [3H]GR 65630, [3H]ICS 205-930 and [3H]quipazine may be useful in determining if 5-HT3 receptor subtypes exist. After injections of 3H-leucine and/or proline in the dorsal cochlear nucleus, most of the labeled endings in the IC contained small, round vesicles (less than 47 nm in diameter) although a very small number of endings with large, round vesicles also were labeled.(ABSTRACT TRUNCATED AT 400 WORDS In the present studies, the ability of [3H]A-317491 to label recombinant human P2X(2/3) and P2X(3) receptors was characterized. Using membranes prepared from 1321N1 cells expressing P2X(2/3) receptors, [3H]A-317491 specifically labeled high-affinity (Kd = 0.9 nM) recognition sites. High-affinity [3H]A-317491 binding was not detected in membrane preparations from native 1321N1 cells or cells expressing homomeric P2X1, P2X2, or P2X3 receptors. Specific [3H]A-317491 P2X3 receptors could only be. For example, the 5-HT1A agonist [3H]8-OH-DPAT also labels a non-5-HT1 site (2,17) and [3H]WB4101 labels alpha-adrenergic receptors (12) in addition to labeling 5-HT1A sites. Our previous work showed that spiroxatrine acted as a full antagonist against 5-HT in the canine basilar artery (5), a preparation which has been proposed as having 5-HTlA-like receptors (6,18). In the rat aorta, a model for 5-HT2 receptor-mediated contractions, it also acted as an antagonist with no evidence.

A sequential double-label 14C- and 3H-2-DG technique

  1. Autoradiographs were prepared from lungs of a newborn Syrian golden hamster exposed continuously to 3H-thymidine for the final 4.5 days of a normal 16 day gestation. Silver grains were counted over nuclei of 1,298 small-granule endocrine cells in 165 neuroepithelial bodies (NEBs) in the right upper lobe and along the left axial bronchus, where nodal NEBs occurred at branch points and internodal NEBs in the airway between them. Nuclei of 1,005 nonendocrine airway epithelial cells were counted.
  2. Europ~n Journ~ of Ph~macology, 48 (1978) 213--218 213 ~d~/Nor~-Holland ~omed~M Fre~ Sho~ communication BENZODIAZEPINE RECEPTORS: LABELING IN INTACT ANIMALS WITH [3H] FLUNITRAZEPAM RAYMOND S.L. CHANG and SOLOMON H. SNYDER Dep~tmen~ of Pharmaco~gy and Expe~mental Therapeutics and Psych~t~ and Behav~l Sc~nce& Joh~ Hopkins Unwersi~ School of Medich~ B~more, Ma~nd 2120~ ~&A. Receded 13 Janua~ 1978.
  3. ary reports have described attempts to label pituitary dopa
  4. g a cyclopropene-like ring, 3H-diazirene.They are isomeric with diazocarbon groups, and like them can serve as precursors for carbenes by loss of a molecule of dinitrogen.For example, irradiation of diazirines with ultraviolet light leads to carbene.
  5. Atomic orbital Mulliken labels C2v D3h D4h Td Oh s px py pz dz2 dx2-y2 dxy dxz dyz D3h A1' x2+y 2, z 2 A2' Rz E' (x,y) (x 2-y2, xy) A1 A2 z E (R x,R y) (xz, yz) Td A1 x2+y 2+z 2 A2 E (2z 2-x2-y2, x 2-y2) T1 (R x,R y,R z) T2 (x,y,z) (xz, yz, xy) Oh A1g x 2+y 2+z2 Eg (2z 2-x2-y2, x 2-y2) T1g (R x,R y,R z) T2g (xz, yz, xy) T1u (x,y,z) D4h A1g x +y 2, z B1g x 2-y B2g xy Eg (R x.

Selective labelling of diazepam-insensitive GABAA

  1. The hormone-binding components of the rat uterine progesterone receptor were investigated by the methods of [3H]R5020 photoaffinity labeling and sodium dodecyl sulfatepolyacrylamide gel electrophoresis analysis. Two specifically labeled peaks were observed at mol wt of 85,600 +/- 1,200 and 109,600 +/- 1,200 (n = 31), resembling the A and B progesterone receptor components previously described in other systems. However, in contrast to the equimolar ratio reported in other systems, the level.
  2. 3H-thymidine labeling index. 3H-thymidine labelling index. 3H-thymidine-labeling index method. 3H]thymidine labeling index. CY+total lymphatic irradiation. dThd labeling index. regimen--total lymphoid irradiation. T lymphocyte immunophenotyping. Tailored Lighting Inc
  3. Epibatidine, a frog skin alkaloid, is a nicotinic receptor agonist with potent analgesic activity. We used in vitro receptor autoradiographic techniques to map binding sites for [3H]epibatidine in rat brain, and those for another nicotinic agonist, [3H]cytisine. Both [3H]epibatidine and [3H]cytisine are excellent radioligands for autoradiography
  4. Rapid Identification of Cysteine-Linked Isoprenyl Groups by Metabolic Labeling with [3 H]Farnesol and [3 H]Geranylgeraniol. Authors; Authors and affiliations; Douglas A. Andres; Dean C. Crick; Brian S. Finlin; Charles J. Waechter; Protocol. 385 Downloads; Part of the Methods in Molecular Biology book series (MIMB, volume 116) Abstract. The posttranslational modification of proteins by the.
  5. ing a cellular process occurring over time by successively exposing the cells to a labeled compound (pulse) and then to the same compound in an unlabeled form (chase).. Mechanism. A selected cell or a group of cells is first exposed to a labeled compound (the pulse) that is to be incorporated into a molecule or.
  6. 2(3H)-Benzothiazolone | C7H5NOS | CID 13625 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities.

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Tritium - Wikipedi

Isotopic Labeling | OPEN

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Labeling of Probes- Overview - Biology Ease3H‐thymidine is a defective tool with which to measure

3H Biomedical A

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PPT - Applications of Molecular Cytogenetics PowerPoint

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Asymmetric trimethine 3H-indocyanine dyes: efficient

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3-Azidodifluoromethyl-3H-diazirin-3-yl group as an all-in

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American Radiolabeled Chemicals - Home Pag

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Distribution of dopaminergic receptors in the primate

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